| Type | Conjugated Primary Antibody |
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| Conjugated with | ALEXA FLUOR® 594 |
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| Host organism | Rabbit (Oryctolagus cuniculus) |
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| Target Protein/Peptide | TRIM25 Ser100 |
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| Specificity | This antibody reacts specifically with TRIM25(Ser100) |
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| Modification | Phosphorylation |
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| Modification site | Ser100 |
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| Clonality | Polyclonal Antibody |
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| Clone | Polyclonal Antibodies |
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| Concentration | 1ug per 1ul |
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| Subcellular locations | N/A |
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| Antigen Source | KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100 |
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| Gene ID | 7706 |
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| Swiss Prot | N/A |
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| Applications | IF(IHC-P) |
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| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
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| Cross reactive species | Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus) |
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| Cross Reactive Species details | No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species. |
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| Background information | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. |
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| Purification method | Purified by Protein A. |
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| Storage | Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months. |
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| Excitation emission | 590nm/617nm |
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| Synonyms | TRIM25phospho S100ï¼›EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite mot containing 25; Tripartite mot containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 estrogen responsive finger protein; ZNF 147; ZNF147. |
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| Also known as | TRIM25(Ser100) Polyclonal Antibody |
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| Other name | Anti-TRIM25(Ser100) Polyclonal |
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| Advisory | Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom. |
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| Properties | For facs or microscopy Alexa 1 conjugate. |
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| Conjugation | Alexa Fluor,ALEXA FLUOR® 594 |
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| Conjugated | Alexa conjugate 1 |
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| Description | This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. |
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| Group | Polyclonals and antibodies |
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| About | Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. |
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