Anti-PDK4 (Polyclonal), ALEXA Fluor 594

Catalog numberGENTObs-0682R-A594
NameAnti-PDK4 (Polyclonal), ALEXA Fluor 594
Price€ 489.00
Size100 microliters
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TypeConjugated Primary Antibody
Conjugated withALEXA FLUOR® 594
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptidePDK4
SpecificityThis antibody reacts specifically with PDK4
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human Pdk4
Gene ID5166
Swiss ProtN/A
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background informationPdk4 (pyruvate dehydrogenase kinase, isozyme 4) mediates glucose oxidation through inhibitory phosphorylation of the pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex (PDC) is a mitochondrial matrix enzyme that plays an key role in aerobic energy metabolism by catalyzing the oxidative decarboxylation of pyruvate to form acetyl-CoA for subsequent breakdown in the Krebs cycle. The enzymatic activity of the mammalian PDC is regulated through phosphorylation and desphosphorylation of the E1?subunit of the complex. The enzymes responsible for the phosphorylation and inactivation of the PDC are pyruvate dehydrogenase kinases (PDK1-4). [TISSUE SPECI FICITY] Ubiquitous; highest levels of expression in heart and skeletal muscle. Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.
Purification methodPurified by Protein A.
StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission590nm/617nm
SynonymsFLJ40832; Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial; Pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoform 4; Pyruvate dehydrogenase kinase isozyme 4 mitochondrial.
Also known asPDK4 Polyclonal Antibody
Other nameAnti-PDK4 Polyclonal
AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
PropertiesFor facs or microscopy Alexa 1 conjugate.
ConjugationAlexa Fluor,ALEXA FLUOR® 594
ConjugatedAlexa conjugate 1
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.