Anti-BOULE (Polyclonal), ALEXA Fluor 594

Catalog numberGENTObs-12880R-A594
NameAnti-BOULE (Polyclonal), ALEXA Fluor 594
Price€ 489.00
Size100 microliters
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TypeConjugated Primary Antibody
Conjugated withALEXA FLUOR® 594
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideBOULE
SpecificityThis antibody reacts specifically with BOULE
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human BOULE
Gene ID66037
Swiss ProtN/A
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background informationSpermatogenesis represents the intricate developmental process of mitotic and meiotic cell divisions that ultimately leads to the production of haploid spermatozoa. BOULE, a member of the human deleted in azoospermia (DAZ) family, functions as a key conserved switch that regulates the progression of germ cells through meiosis in man. BOULE is an RNA-binding protein that regulates the expression of twine, a Cdc25 phosphatase, which promotes progression through meiosis. BOULE is expressed not only in the testis, but also in the nervous system, where it may play a role in neural communication. Mutations in the BOULE gene are be associated with male infertility, and the relative proportions of the three BOULE isoforms (B1, B2 and B3) may function as predictive markers for meiotic efficiency.
Purification methodPurified by Protein A.
StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission590nm/617nm
SynonymsBol Drosophila boule homolog like; Bol; Bol boule like; Bol, boule like Drosophila; bol, boule-like Drosophila; Boll; BOLL_HUMAN; BOULE; Boule like; BOULE, Drosophila, homolog of; Protein boule like; Protein boule-like; Putative uncharacterized protein BOLL.
Also known asBOULE Polyclonal Antibody
Other nameAnti-BOULE Polyclonal
AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
PropertiesFor facs or microscopy Alexa 1 conjugate.
ConjugationAlexa Fluor,ALEXA FLUOR® 594
ConjugatedAlexa conjugate 1
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.