Anti-AKT1/2/3 (Ser472/Ser473/Ser474), ALEXA Fluor 594

Catalog numberGENTObs-0876R-A594
NameAnti-AKT1/2/3 (Ser472/Ser473/Ser474), ALEXA Fluor 594
Price€ 516.00
Size100 microliters
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TypeConjugated Primary Antibody
Conjugated withALEXA FLUOR® 594
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideAKT1/2/3 (Ser472/Ser473/Ser474)
SpecificityThis antibody reacts specifically with AKT1/2/3 (Ser472/Ser473/Ser474)
ModificationPhosphorylation
Modification siteSer473
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsCytoplasm, Nucleus
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Gene ID24185
Swiss ProtP47196
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background informationAKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Purification methodPurified by Protein A.
StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission590nm/617nm
SynonymsAkt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Also known asAKT1/2/3 (Ser472/Ser473/Ser474) Antibody
Other nameAnti-AKT1/2/3 (Ser472/Ser473/Ser474)
AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
PropertiesFor facs or microscopy Alexa 1 conjugate.
ConjugationAlexa Fluor,ALEXA FLUOR® 594
ConjugatedAlexa conjugate 1
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.