CDC27 (Thr244) Antibody, ALEXA FLUOR 647

Catalog numberbs-5268R-A647
NameCDC27 (Thr244) Antibody, ALEXA FLUOR 647
Price€ 350.15
Size100ul
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Long nameCDC27 (Thr244) Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
Also known asAnti-CDC27 Thr244 PAb ALEXA FLUOR 647
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 647
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCDC27 Thr244
SpecificityThis is a highly specific antibody against CDC27 Thr244.
ModificationPhosphorylation
Modification SiteThr244
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244
Gene ID Number996
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenThe protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission650nm/665nm
SynonymsANAPC3; Anaphase Promoting Complex 3; Anaphase promoting complex protein 3; Anaphase Promoting Complex Subunit 3; Anaphase-promoting complex subunit 3; APC 3; APC3; APC-3; Cdc 27; Cdc27; Cdc-27; CDC27 homolog; CDC27_HUMAN; CDC27Hs; Cell division cycle 27; Cell division cycle protein 27 homolog; H NUC; H-NUC; HNUC; NUC2 homolog.
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-CDC27 Thr244 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR 647
French translationanticorps