| Long name | GRAP Polyclonal Antibody, ALEXA FLUOR 555 Conjugated |
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| Also known as | Anti-GRAP PAb ALEXA FLUOR 555 |
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| Category | Conjugated Primary Antibodies |
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| Conjugated with | ALEXA FLUOR® 555 |
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| Host Organism | Rabbit (Oryctolagus cuniculus) |
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| Target Antigen | GRAP |
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| Specificity | This is a highly specific antibody against GRAP. |
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| Modification | Unmodified |
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| Modification Site | None |
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| Clonality | Polyclonal |
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| Clone | Polyclonal antibody |
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| Concentration | 1ug per 1ul |
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| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GRAP |
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| Tested applications | IF(IHC-P) |
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| Recommended dilutions | IF(IHC-P)(1:50-200) |
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| Crossreactivity | Human, Mouse, Rat |
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| Cross-reactive species details | Due to limited amount of testing and knowledge, not every possible cross-reactivity is known. |
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| Background of the antigen | Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences. For instance, the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains. Members include GRB2, GRB7, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein). While GRB10 and GRB14 are most closely related to GRB7, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2. The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, c-Kit and the erythropoietin receptor (EpoR). Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain. |
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| Purification | Purified by Protein A. |
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| Storage conditions | Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year. |
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| Excitation emission | 553nm/568nm |
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| Synonyms | Grap; GRAP_HUMAN; GRB2 related adaptor protein; GRB2-related adapter protein; Growth factor receptor bound protein 2 related adaptor protein; MGC64880; OTTHUMP00000065766. |
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| Properties | For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. |
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| Conjugation | Alexa Fluor,ALEXA FLUOR 555 |
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| French translation | anticorps |
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