PKC gamma (Thr514) Antibody, ALEXA FLUOR 488

Catalog numberbs-3729R-A488
NamePKC gamma (Thr514) Antibody, ALEXA FLUOR 488
Price€ 350.15
Size100ul
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Long namePKC gamma (Thr514) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known asAnti-PKC gamma Thr514 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 488
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPKC gamma Thr514
SpecificityThis is a highly specific antibody against PKC gamma Thr514.
ModificationPhosphorylation
Modification SiteThr514
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range500-540/697
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human PKC gamma around the phosphorylation site of Thr514
Gene ID Number5582
Swiss ProtP05129
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq, Jul 2008]
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission499nm/519nm
SynonymsPKCC; PKCG; SCA14; PKC-gamma; Protein kinase C gamma type; PRKCG
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-PKC gamma Thr514 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor
French translationanticorps