Wee1(Ser123) Antibody, ALEXA FLUOR 647

Catalog numberbs-5218R-A647
NameWee1(Ser123) Antibody, ALEXA FLUOR 647
Price€ 350.15
Size100ul
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Long nameWee1(Ser123) Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
Also known asAnti-Wee1 Ser123 PAb ALEXA FLUOR 647
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 647
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenWee1 Ser123
SpecificityThis is a highly specific antibody against Wee1 Ser123.
ModificationPhosphorylation
Modification SiteSer123
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range100-150/646
Subcellular locationNucleus
SourceKLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV]
Gene ID Number7465
Swiss ProtP30291
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission650nm/665nm
SynonymsWEE1A; WEE1hu; Wee1-like protein kinase; Wee1A kinase; WEE1
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-Wee1 Ser123 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR 647
French translationanticorps