HCST Antibody, ALEXA FLUOR 555

Catalog numberbs-15430R-A555
NameHCST Antibody, ALEXA FLUOR 555
Price€ 380.00
Size100ul
  Get from shop
Long nameHCST Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
Also known asAnti-HCST PAb ALEXA FLUOR 555
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 555
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenHCST
SpecificityThis is a highly specific antibody against HCST.
ModificationUnmodified
Modification SiteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range1-50/93
Subcellular locationExtracellular
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human HCST
Gene ID Number10870
Swiss ProtQ9UBK5
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenTransmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission553nm/568nm
SynonymsDAP10; KAP10; PIK3AP; Hematopoietic cell signal transducer; DNAX-activation protein 10; Membrane protein DAP10; Transmembrane adapter protein KAP10; HCST; UNQ587/PRO1157
PropertiesFor facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR 555
French translationanticorps