SLK (Tyr530) Antibody, ALEXA FLUOR 488

Catalog numberbs-5332R-A488
NameSLK (Tyr530) Antibody, ALEXA FLUOR 488
Price€ 350.15
Size100ul
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Long nameSLK (Tyr530) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known asAnti-SLK Tyr530 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 488
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenSLK Tyr530
SpecificityThis is a highly specific antibody against SLK Tyr530.
ModificationPhosphorylation
Modification SiteTyr530
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human FYN around the phosphorylation site of Tyr530
Gene ID Number2534
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenFyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1. The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission499nm/519nm
SynonymsFyn phospho Y530; p-Fyn phospho Y530; C syn protooncogene; FYN oncogene related to SRC FGR YESï¼› MGC45350ï¼› OKT3 induced calcium influx regulatorï¼› P59 FYN; Protein tyrosine kinase fyn; Proto oncogene tyrosine protein kinase fyn; Protooncogene Syn; SLK; Src like kinase; Src yes related novel gene; Src/yes related novel gene; SYN; FYN; Tyrosine kinase p59fyn T; Tyrosine kinase p59fynT.
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-SLK Tyr530 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor
French translationanticorps