BCAR1 (Tyr249) Antibody, ALEXA FLUOR 555

Catalog numberbs-12574R-A555
NameBCAR1 (Tyr249) Antibody, ALEXA FLUOR 555
Price€ 350.15
Size100ul
  Get from shop
Long nameBCAR1 (Tyr249) Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
Also known asAnti-BCAR1 Tyr249 PAb ALEXA FLUOR 555
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 555
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenBCAR1 Tyr249
SpecificityThis is a highly specific antibody against BCAR1 Tyr249.
ModificationPhosphorylation
Modification SiteTyr249
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr249
Gene ID Number9564
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenp130 represents one of several known substrates for v-Crk encoded p47. p130 Cas (for Crk-associated substrate) exhibits a high level of tyrosine phosphorylation and is tightly associated with v-Crk, suggesting a role in v-Crk-mediated cell signaling. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission553nm/568nm
SynonymsBCAR1 phospho Y249; BCAR1phospho Y249; p-BCAR1p-Tyr249; Breast cancer anti estrogen resistance 1 protein; BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; P130CAS.
PropertiesFor facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR 555
French translationanticorps