IKK beta(Tyr199) Antibody, ALEXA FLUOR 555

Catalog numberbs-3232R-A555
NameIKK beta(Tyr199) Antibody, ALEXA FLUOR 555
Price€ 350.15
Size100ul
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Long nameIKK beta(Tyr199) Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
Also known asAnti-IKK beta Tyr199 PAb ALEXA FLUOR 555
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 555
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenIKK beta Tyr199
SpecificityThis is a highly specific antibody against IKK beta Tyr199.
ModificationPhosphorylation
Modification SiteTyr199
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range180-230/756
Subcellular locationCytoplasm, Nucleus
SourceKLH conjugated synthetic phosphopeptide derived from human IKK beta around the phosphorylation site of Tyr199
Gene ID Number3551
Swiss ProtO14920
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenSerine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission553nm/568nm
SynonymsIKK2; IKKB; IMD15; NFKBIKB; IKK-beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IkBKB; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
PropertiesFor facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR 555
French translationanticorps