Histone H3 (Ser28) Antibody, ALEXA FLUOR 488

Catalog numberbs-5385R-A488
NameHistone H3 (Ser28) Antibody, ALEXA FLUOR 488
Price€ 350.15
Size100ul
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Long nameHistone H3 (Ser28) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known asAnti-Histone H3 Ser28 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 488
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenHistone H3 Ser28
SpecificityThis is a highly specific antibody against Histone H3 Ser28.
ModificationPhosphorylation
Modification SiteSer28
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser28
Gene ID Number3020
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission499nm/519nm
SynonymsHistone H3Ser28; Histone H3Ser28; Histone H3p-Ser28; Histone H3S28; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_HUMAN; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Histone H3 Ser28 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor
French translationanticorps