Histone H3 (Tri Methyl K27) Antibody, ALEXA FLUOR 350

Catalog numberbs-3773R-A350
NameHistone H3 (Tri Methyl K27) Antibody, ALEXA FLUOR 350
Price€ 350.15
Size100ul
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Long nameHistone H3 (Tri Methyl K27) Antibody, ALEXA FLUOR 350 Conjugated
Also known asAnti-Histone H3 Tri Methyl K27 PAb ALEXA FLUOR 350
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 350
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenHistone H3 Tri Methyl K27
SpecificityThis is a highly specific antibody against Histone H3 Tri Methyl K27.
ModificationMethylation
Modification SiteTri Methyl K27
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic methyl peptide derived from human Histone H3 around methylation site of Lys27
Gene ID Number3020
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenModulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission343nm/442nm
SynonymsHistone H3, H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene.
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR 350
DescriptionA methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
French translationanticorps