Rat Anti-Chicken GRP94 Monoclonal IgG2a, Clone 9G10

Catalog numberSMC-105B-A488
NameRat Anti-Chicken GRP94 Monoclonal IgG2a, Clone 9G10
Price€ 406.00
Size200 µg
  Get from shop
Clone9G10
ImmunogenPurified Grp94 isolated from chicken oviducts
Antibody's full descriptionRat Anti-Chicken GRP94 Monoclonal IgG2a Antibody, Clone: 9G10: ATTO 488
Antibody's categoryMonoclonal Antibodies
Antibody's other nameHSP90B1 Antibody, GP96 Antibody, TRA1 Antibody, ECGP Antibody
Raised inRat
Antibody's targetGRP94
Primary research fieldsCancer, Heat Shock, Cell Signaling, Trafficking
BrandnameNone
Antibodies' applicationsWB, ICC/IF, IP, FCM
Antibody's reactivityHuman, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig (Cavia porcellus), Hamster, Horse, Monkey, Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis)
Antibody's dilutionsWB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
PurityProtein G Purified
Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
Antibody's concentration1 mg/ml
Antibody's specificityDetects ~98kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
Storage recommendations-20ºC
Shipping recommendationsBlue Ice or 4ºC
Antibody certificate of analysis0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Antibody in cellEndoplasmic Reticulum, Endoplasmic Reticulum Lumen , Melanosome
Tissue specificitySee included datasheet or contact our support service
Scientific contextGrp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
Bibliography1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128. 2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98. 3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262:8875-8883. 4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9):5643-5649. 5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338. 6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269. 7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637. 8. Allen S. et al. (2000) Blood 96(2): 560-568. 9. Sato K et al. (2001) Blood 98(6): 1852-1857. 10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5):371-378. 11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858. 12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386. 13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104. 14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
Released date1-Jun-2007
Tested applicationsTo be tested
Tested reactivityTo be tested
NCBI numberNP_003290.1
Gene number7184
Protein numberP14625
Antibody's datasheetContact our support service
Representative figure link | |
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (SMC-105). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (SMC-105) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (SMC-105). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (SMC-105) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Western Blot analysis of Human HeLa cell lysates showing detection of GRP94 protein using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (SMC-105). Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (SMC-105) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat. Rat Anti-GRP94 Antibody [9G10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-105) Rat Anti-GRP94 Antibody [9G10] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-105) Rat Anti-GRP94 Antibody [9G10] used in Western Blot (WB) on Human HeLa cell lysates (SMC-105)
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.2
Stock availabilityIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PropertiesChickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin nameRattus norvegicus