Rabbit Anti-Human BDNF Polyclonal: ATTO 390

Catalog numberSPC-703D-A390
NameRabbit Anti-Human BDNF Polyclonal: ATTO 390
Price€ 398.00
Size100 µg
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ImmunogenSynthetic peptide from the N-terminal of human BDNF
Antibody's targetHuman BDNF
Antibody's full descriptionRabbit Anti-Human BDNF Polyclonal
Primary research fieldsNeuroscience, Neurodegeneration, Alzheimer's Disease, Cardiovascular System, Atherosclerosis, ALS Disease
Antibody's categoryPolyclonal Antibodies
Antibody's other nameAbrineurin Antibody, ANON2 Antibody, BDNF_Human Antibody, Brain Derived Neurotrophic Factor Antibody, MGC34632 Antibody
Verified applicationsWB, ICC/IF
Raised inRabbit
Antibody's reactivityHuman, Mouse
Antibody's recommended dilutions for useWB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody's purified fromPeptide Affinity Purified
Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
Antibody's concentration1 mg/ml
Antibody's specificityPredicted molecular weight at ~27.9kDa.
Storage recommendations-20°C
Shipping recommendationsBlue Ice or 4°C
Certificate of analysisA 1:1000 dilution of SPC-703 was sufficient for detection of BDNF on 293T Rapamycin-treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cellSecreted
Tissue specificitySee included datasheet or contact our support service.
Scientific contextBrain-derived neurotrophic factor, also known as BDNF, is a protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. Neurotrophic factors are found in the brain and the periphery. The effects of BDNF on motor neurons might be useful in treating patients with motor neuropathies and ALS (1-3).
Bibliography1. Wetmore C., Ernfors P., Persson H., Olson L. (1990) Exp. Neurol. 109(2): 141-152. 2. Thoenen H., Zafra F., Hengerer B., Lindholm D. (1991) Ann NY Acad Sci. 640: 86-90. 3. Chun H.S., Son J.J., Son J.H. (2000) Neuroreport. 11(3): 511-514.
Released date1/Oct/2015
NCBI numberNP_001137277.1
Gene number627
Protein numberP23560
PubMed numberRefer to PubMed
Tested applicationsTo be tested
Tested reactivityTo be tested
Antibody's datasheetContact our support service to receive datasheet or other technical documentation.
Representative figure link | |
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody (SPC-703). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (SPC-703) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite. | Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (SPC-703). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (SPC-703) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda. | Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (SPC-703). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (SPC-703) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda. Rabbit Anti-BDNF Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-703) | Rabbit Anti-BDNF Antibody used in Western blot (WB) on HeLa and 293T cell lysates (SPC-703) | Rabbit Anti-BDNF Antibody used in Western blot (WB) on Brain (SPC-703)
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.1
Stock availabilitIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestStressMark antibodies supplies antibodies that are for research of human proteins.
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin nameOryctolagus cuniculus