Rabbit Anti-Rat UT-A1 Polyclonal: Unconjugated

Catalog numberSPC-406D
NameRabbit Anti-Rat UT-A1 Polyclonal: Unconjugated
Price€ 359.00
Size100 µg
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ImmunogenProduced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)
Antibody's targetRat UT-A1
Antibody's full descriptionRabbit Anti-Rat UT-A1 Polyclonal
Primary research fieldsNeuroscience, Pumps/Transporters, Urea Transporters
Antibody's categoryPolyclonal Antibodies
Antibody's other nameSLC14A2 Antibody, FLJ16167 Antibody, hUT-A6 Antibody, HUT2 Antibody, kidney Antibody, MGC119566 Antibody, MGC119567 Antibody, Slc14a2 Antibody, Solute carrier family 14 (urea transporter) Antibody, member 2 Antibody, Solute carrier family 14 member 2 Antibody, Urea transporter 2 Antibody, Urea transporter Antibody, Urea transporter kidney Antibody, UT-A2 Antibody, UT2 Antibody, UT2_HUMAN Antibody, UTA Antibody, UTR Antibody, UT1 Antibody, UTA1 Antibody
Verified applicationsWB, ICC/IF
Raised inRabbit
Antibody's reactivityRat, Mouse
Antibody's recommended dilutions for useWB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody's purified fromProtein A Purified
Recommended buffer for storagePBS, 50% glycerol, 0.09% sodium azide
Antibody's concentration1 mg/ml
Antibody's specificityDetects ~97 and 127kDa.
Storage recommendations-20°C
Shipping recommendationsBlue Ice or 4°C
Certificate of analysis1 µg/ml of SPC-406 was sufficient for detection of UT-A1 in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cellCell Membrane, Apical Cell Membrane
Tissue specificitySee included datasheet or contact our support service.
Scientific contextUT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2) (1, 2).
Bibliography1. Nielsen S., et al. (1996) Proc Natl Acad Sci U S A. 93(11): 5495-500. 2. Smith C.P. (2009) Exp Physiol. 94(2): 180-185.
Released date11/Jun/2010
NCBI numberNP_062220
Gene number54302
Protein numberQ62668
PubMed numberRefer to PubMed
Tested applicationsTo be tested
Tested reactivityTo be tested
Antibody's datasheetContact our support service to receive datasheet or other technical documentation.
Representative figure legendWestern blot analysis of Rat Inner medulla showing detection of UT-A1 protein using Rabbit Anti-UT-A1 Polyclonal Antibody (SPC-406). Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody (SPC-406) at 1:1000. Rabbit Anti-UT-A1 Antibody used in Western blot (WB) on Rat Inner medulla (SPC-406)
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.1
Stock availabilitIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin nameOryctolagus cuniculus,Rattus norvegicus
Conjugationnot conjugated