Rabbit Anti-Yeast AHA1p Polyclonal: Alkaline Phosphatase

Catalog numberSPC-204D-ALP
NameRabbit Anti-Yeast AHA1p Polyclonal: Alkaline Phosphatase
Price€ 369.00
Size100 µl
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ImmunogenYeast Aha1 raised against peptide (CESQVKSNYTRGNQK)-KLH fusion
Antibody's targetYeast AHA1p
Antibody's full descriptionRabbit Anti-Yeast AHA1p Polyclonal
Primary research fieldsCancer, Heat Shock, Cell Signaling, Trafficking, Chaperones
Antibody's categoryPolyclonal Antibodies
Antibody's other nameActivator of 90 kDa heat shock protein ATPase homolog 1 Antibody, Aha1 Antibody, SHSA1 Antibody, HSPC322 Antibody, p38 Antibody
Verified applicationsWB
Raised inRabbit
Antibody's reactivityYeast
Antibody's recommended dilutions for useWB (1:2500); optimal dilutions for assays should be determined by the user.
Antibody's purified fromRabbit Antiserum
Recommended buffer for storageRabbit Antiserum, PBS, 50% glycerol
Antibody's concentrationSee included datasheet or contact our support service
Antibody's specificityDetects ~ 38kDa. Can run up to 45kDa on SDS Page.
Storage recommendations-20°C
Shipping recommendationsBlue Ice or 4°C
Certificate of analysis0.5 µg/ml of SPC-204 was sufficient for detection of Aha1p in 20 µg of W303 yeast lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cellCytoplasm, Endoplasmic Reticulum
Tissue specificitySee included datasheet or contact our support service.
Scientific contextAha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Bibliography1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
Released date2/Apr/2013
NCBI numberNP_010500.3
Gene number851800
Protein numberQ12449
PubMed numberRefer to PubMed
Tested applicationsTo be tested
Tested reactivityTo be tested
Antibody's datasheetContact our support service to receive datasheet or other technical documentation.
Representative figure linkNo Data Available
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.1
Stock availabilitIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin nameOryctolagus cuniculus