Rabbit Anti-Yeast STI1P (HOP) Polyclonal: Unconjugated

Catalog numberSPC-203D
NameRabbit Anti-Yeast STI1P (HOP) Polyclonal: Unconjugated
Price€ 327.00
Size100 µl
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ImmunogenYeast Sti1p raised against peptide(CDINQSNSMPKEPET)-KLH fusion
Antibody's targetYeast HOP
Antibody's full descriptionRabbit Anti-Yeast STI1P (HOP) Polyclonal
Primary research fieldsCancer, Heat Shock
Antibody's categoryPolyclonal Antibodies
Antibody's other nameSTIP1 Antibody, Sti1 yeast Antibody, Hop Antibody, HSC70/HSP90 organizing protein Antibody, Epididymis secretory sperm binding protein Li 94n antibody, HEL S 94n antibody, Hop antibody, HSC70/HSP90 organizing protein antibody, HSC70/HSP90-organizing protein antibody, IEF SSP 3521 antibody, NY REN 11 antigen antibody, P60 antibody, Renal carcinoma antigen NY-REN-11 antibody, STI1 antibody, STI1L antibody, STIP1 antibody, Stress induced phosphoprotein 1 antibody, Stress-induced-phosphoprotein 1 antibody, Transformation sensitive protein IEF SSP 3521 antibody, Transformation-sensitive protein IEF SSP 3521 antibody
Verified applicationsWB, ELISA, FCM
Raised inRabbit
Antibody's reactivityMouse, Yeast
Antibody's recommended dilutions for useWB (1:2500); optimal dilutions for assays should be determined by the user.
Antibody's purified fromRabbit Antiserum
Recommended buffer for storageRabbit Antiserum, PBS, 50% glycerol
Antibody's concentrationSee included datasheet or contact our support service
Antibody's specificityDetects ~ 66kDa.
Storage recommendations-20°C
Shipping recommendationsBlue Ice or 4°C
Certificate of analysis0.5 µg/ml of SPC-203 was sufficient for detection of Sti1p (HOP) in 20 µg of W303 yeast lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cellCytoplasm, Nucleus
Tissue specificitySee included datasheet or contact our support service.
Scientific contextThe stress inducible protein Sti1p is also commonly known as HSP70-HSP90 organization protein (HOP). It is located in diverse cellular regions and can move between the cytoplasm and the nucleus. It functions to reversibly link together the protein chaperones HSP70 and HSP90. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. HSP70 binding has been localized to TRP1 and sp90 binding have been localized to TPR2a (1). It has also been found to modulate the chaperone activities of the linked proteins and possible interacts with other chaperones and proteins. It has also been found to participate in other complexes besides the HSP70/HSP90 one (2). HOP is closely related to human 63kDa protein that is sensitive to simian virus SV40 transformation, and is related to the yeast heat-shock- responsive STI1 gene product (3, 4).
Bibliography1. Flom G., Behal R.H., Rosen L., Cole D.G., Johnson J.L. (2007) Biochem J. 404(1): 159-167. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt3): 789-796. 3. Honore B.H., et al. (1992) J Biol Chem. 267: 8485- 8491. 4. Nicolet C.M., et al. (1989) Mol Cell Bio. 9: 3638-3646.
Released date2/Apr/2013
NCBI numberNP_014670.1
Gene number854192
Protein numberP15705
PubMed number27665558|27665558
Tested applicationsELISA|Flow Cytometry
Tested reactivityMouse|Mouse
Antibody's datasheetContact our support service to receive datasheet or other technical documentation.
Representative figure linkNo Data Available
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.1
Stock availabilitIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin nameOryctolagus cuniculus
Conjugationnot conjugated