Rabbit Anti-Human Bassoon Polyclonal: Biotin

Catalog numberSPC-198D-BI
NameRabbit Anti-Human Bassoon Polyclonal: Biotin
Price€ 392.00
Size100 µg
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ImmunogenNM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein
Antibody's targetHuman Bassoon
Antibody's full descriptionRabbit Anti-Human Bassoon Polyclonal
Primary research fieldsNeuroscience, Cell Structure, Pre-Synaptic Markers
Antibody's categoryPolyclonal Antibodies
Antibody's other nameBSN Antibody, ZNF231 Antibody, Neuronal double zine finger protein Antibody
Verified applicationsWB, IHC
Raised inRabbit
Antibody's reactivityHuman, Mouse, Rat
Antibody's recommended dilutions for useWB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.
Antibody's purified fromProtein A Purified
Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
Antibody's concentration1 mg/ml
Antibody's specificityDetects ~420kDa. Multiple isoforms can be detected.
Storage recommendations-20°C
Shipping recommendationsBlue Ice or 4°C
Certificate of analysis1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
Antibody in cellCytoplasm, Cell Junction, Synapse
Tissue specificitySee included datasheet or contact our support service.
Scientific contextBassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors (1).
Bibliography1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008–1029.
Released date22/Mar/2011
NCBI numberNP_003449.2
Gene number8927
Protein numberQ9UPA5
PubMed numberRefer to PubMed
Tested applicationsTo be tested
Tested reactivityTo be tested
Antibody's datasheetContact our support service to receive datasheet or other technical documentation.
Representative figure link |
Representative figure legendImmunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ. | Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:1000. Rabbit Anti-Bassoon Antibody used in Immunohistochemistry (IHC) on Mouse Muscle (SPC-198) | Rabbit Anti-Bassoon Antibody used in Western blot (WB) on Mouse, Rat brain cell lysates (SPC-198)
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.1
Stock availabilitIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestStressMark antibodies supplies antibodies that are for research of human proteins.
PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin nameOryctolagus cuniculus