Rabbit Anti-Rat SOD (Cu/Zn) Polyclonal: PE/ATTO 594

Catalog numberSPC-115D-P594
NameRabbit Anti-Rat SOD (Cu/Zn) Polyclonal: PE/ATTO 594
Price€ 417.00
Size100 µg
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ImmunogenRat Cu/Zn SOD
Antibody's targetRat SOD1
Antibody's full descriptionRabbit Anti-Rat SOD (Cu/Zn) Polyclonal
Primary research fieldsCancer, Oxidative Stress, Cell Signaling, Trafficking, Chaperones, Neuroscience, Neurodegeneration, ALS Disease
Antibody's categoryPolyclonal Antibodies
Antibody's other nameSuperoxide dismutase1 Antibody, ALS1 Antibody, IPOA Antibody, SOD1 Antibody, SOD2 Antibody, SODC Antibody
Verified applicationsWB, IHC, ICC/IF, IP, ELISA
Raised inRabbit
Antibody's reactivityHuman, Rat, Mouse, Bovine
Antibody's recommended dilutions for useWB (1:2000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Antibody's purified fromProtein A Purified
Recommended buffer for storagePBS pH7.0, 50% glycerol, 0.09% sodium azide
Antibody's concentration1 mg/ml
Antibody's specificityDetects ~23kDa (human) and ~19kDa (other species).
Storage recommendations-20°C
Shipping recommendationsBlue Ice or 4°C
Certificate of analysis0.5 µg/ml of SPC-115 was sufficient for detection of Cu/Zn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Antibody in cellCytoplasm
Tissue specificitySee included datasheet or contact our support service.
Scientific contextSuperoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Bibliography1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
Released date1/Jun/2007
NCBI numberNP_058746.1
Gene number24786
Protein numberP07632
PubMed number23089690|20034236|19493551
Tested applicationsWestern Blot|Western Blot |Western Blot
Tested reactivityRat|Rat|Mouse
Antibody's datasheetContact our support service to receive datasheet or other technical documentation.
Representative figure link | |
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (SPC-115). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (SPC-115) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite. | Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody (SPC-115). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody (SPC-115) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (SPC-115). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody (SPC-115) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite. Rabbit Anti-SOD (Cu/Zn) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-115) | Rabbit Anti-SOD1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-115) | Rabbit Anti-SOD (Cu/Zn) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-115)
Warning informationNon-hazardous
Country of productionCanada
Total weight (kg)1.4
Net weight (g)0.1
Stock availabilitIn Stock
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
GroupPolyclonals and antibodies
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin nameOryctolagus cuniculus,Rattus norvegicus