pRb (7E6) Monoclonal Antibody

Catalog numberbsm-50229M
NamepRb (7E6) Monoclonal Antibody
Price€ 362.00
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Long namepRb (7E6) Monoclonal Antibody
Also known aspRb (7E6) Monoclonal Antibody
CategoryPrimary Antibodies
Target AntigenpRb(retinoblastoma protein)
SpecificityThis is a highly specific antibody against pRb(retinoblastoma protein).
Modification site(s)Unmodified antibody
Clone number7000000
Concentration1ug per 1ul
SourceRecombinant human protein purified from E.coli
Gene ID number5925
Swiss ProtP06400
Tested ApplicationsWB
Recommended dilutionsWB (0.5 ug/mL) IP (2.0 ug)
Cross reactivityHuman
Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the target antigenKey regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Purification methodPurified by Protein G.
Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Synonym namesRB; pRb; OSRC; pp110; p105-Rb; PPP1R130; Retinoblastoma-associated protein; RB1
PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
French translationanticorps