Caspase-9 (Tyr153) Antibody

Catalog numberbs-3083R
NameCaspase-9 (Tyr153) Antibody
Price€ 281.00
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Long nameCaspase-9 (Tyr153) Primary Polyclonal Antibody
Also known asCaspase-9 (Tyr153) PAb
CategoryPrimary Antibodies
Target AntigenCaspase-9 Tyr153
SpecificityThis is a highly specific antibody against Caspase-9 Tyr153.
Modification site(s)Tyr153
ClonalityPolyclonal antibody
Clone numberPolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human Caspase 9 around the phosphorylation site of Tyr153
Gene ID number842
Tested ApplicationsWB, IHC-P, IF(IHC-P)
Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Cross reactivityHuman
Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the target antigenCaspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.
Purification methodThis antibody was purified via Protein A.
Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Synonym namesAPAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; CASP 9; CASP9; Caspase 9 Dominant Negative; Caspase 9 precursor; Caspase 9c; Caspase9; EC 3.4.22.; ICE LAP6; ICE like apoptotic protease 6; MCH6 antibody RNCASP9; CASP9_HUMAN.
PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
DescriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
French translationanticorps