Caspase 8 Antibody

Catalog numberbs-0052R
NameCaspase 8 Antibody
Price€ 263.00
Size0.1ml
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Long nameCaspase 8 Primary Polyclonal Antibody
Also known asCaspase 8 PAb
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenCaspase 8
SpecificityThis is a highly specific antibody against Caspase 8.
Modification(s)None
Modification site(s)Unmodified antibody
ClonalityPolyclonal antibody
Clone numberPolyclonal antibody
Concentration1ug per 1ul
Immunogen range430-482/482
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10
Gene ID number12370
Swiss ProtO89110
Tested ApplicationsWB, IHC-P, IF(IHC-P), IF(ICC)
Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Cross reactivityHuman, Mouse, Rat
Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the target antigenMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Purification methodThis antibody was purified via Protein A.
Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Synonym namesMACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8
PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
DescriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
French translationanticorps