IKB epsilon (Ser157) Antibody, ALEXA FLUOR 488

Catalog numberbs-5517R-A488
NameIKB epsilon (Ser157) Antibody, ALEXA FLUOR 488
Price€ 350.15
Size100ul
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Long nameIKB epsilon (Ser157) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known asAnti-IKB epsilon Ser157 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 488
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenIKB epsilon Ser157
SpecificityThis is a highly specific antibody against IKB epsilon Ser157.
ModificationPhosphorylation
Modification SiteSer157
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human NFKBIE around the phosphorylation site of Ser157
Gene ID Number4794
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenNFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission499nm/519nm
SynonymsNFKBIE phospho S157; IKB epsilon Ser157; IKB epsilon S; p-IKB epsilon Ser157; p-IKB epsilon S157; I kappa B epsilon; IkappaBepsilon; IkB E; IKBE; IKB epsilon; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NFkappa BIE; NFkappaB inhibitor epsilon; NFKBIE; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon; OTTHUMP00000016522; Slc35b2; solute carrier family 35, member B2.
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the IKB epsilon (Ser157) Antibody, has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor
French translationanticorps