DNA Ligase IV/LIG4 Antibody, ALEXA FLUOR 488

Catalog numberbs-1677R-A488
NameDNA Ligase IV/LIG4 Antibody, ALEXA FLUOR 488
Price€ 332.75
Size100ul
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Long nameDNA Ligase IV/LIG4 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known asAnti-DNA Ligase IV/LIG4 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 488
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenDNA Ligase IV/LIG4
SpecificityThis is a highly specific antibody against DNA Ligase IV/LIG4.
ModificationUnmodified
Modification SiteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range60-110/911
Subcellular locationNucleus
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DNA Ligase IV
Gene ID Number3981
Swiss ProtP49917
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Mouse, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenEfficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission499nm/519nm
SynonymsLIG4S; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
PropertiesFor facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the DNA Ligase IV/LIG4 Antibody, has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor
French translationanticorps