p95 NBS1 (Ser343) Antibody, ALEXA FLUOR 594

Catalog numberbs-3718R-A594
Namep95 NBS1 (Ser343) Antibody, ALEXA FLUOR 594
Price€ 350.15
Size100ul
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Long namep95 NBS1 (Ser343) Polyclonal Antibody, ALEXA FLUOR 594 Conjugated
Also known asAnti-p95 NBS1 Ser343 PAb ALEXA FLUOR 594
CategoryConjugated Primary Antibodies
Conjugated withALEXA FLUOR® 594
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigenp95 NBS1 Ser343
SpecificityThis is a highly specific antibody against p95 NBS1 Ser343.
ModificationPhosphorylation
Modification SiteSer343
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range320-370/754
Subcellular locationNucleus
SourceKLH conjugated synthetic phosphopeptide derived from human NBS1 around the phosphorylation site of Ser343
Gene ID Number4683
Swiss ProtO60934
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityHuman, Rat
Cross-reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigenComponent of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
PurificationPurified by Protein A.
Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission590nm/617nm
SynonymsATV; NBS; P95; NBS1; AT-V1; AT-V2; Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1; NBN
PropertiesFor facs or microscopy Alexa 1 conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
ConjugationAlexa Fluor,ALEXA FLUOR® 594
ConjugatedAlexa conjugate 1
French translationanticorps